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KMID : 1094020080250050340
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Journal of Veterinary Clinics
2008 Volume.25 No. 5 p.340 ~ p.345
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IgG Humoral Immune Response to Extract Proteins of Malassezia Pachydermatis Isolated from a Dog with Atopic Dermatitis (Ad)
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Kim Eun-Tae
Kim Ha-Jung
Lim Chae-Young
Park Hee-Myung
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Abstract
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Malassezia pachydermatis (M. pachydermatis) is a component of the normal cutaneous flora of the dog and atopic dermatitis (AD) is one of the most common diseases associated with Malassezia overgrowth in dogs. The purpose of this study was to investigate the humoral response (IgG) to extracts of M. pachydermatis of in a dog with AD. We used Western immunoblotting to identify allergens of M. pachydermatis. Gel electrophoresis of extracts proteins and immunoblotting of sera samples in both an atopic dog and a non-atopic dog were compared. Proteins of 18, 21, 26, 32, 34, 38, 40, 42, 46, 58, 64, 75, 85, and 120 kDa were observed in a serum of atopic dog. However, when serum of a non-atopic dog was used, protein bands were not identified except binding in 50 kDa protein. The results of this study indicate that atopic dogs with M. pachydermatis dermatitis may induce IgG response and also suggest that humoral response to M. pachydermatis could be important in pathogenesis of AD in dogs. However, further studies are required to identify roles of humoral response to M. pachydermatis in canine AD.
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KEYWORD
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atopic dermatitis, dog, IgG, humoral response, Malassezia pachydermatis
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